New saccharogenic determination of alpha-amylase in serum and urine.
نویسنده
چکیده
I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed before measurement of amylase activity is begun.
منابع مشابه
Modified automated saccharogenic determination of serum and urinary amylase activity.
The automated rrvethod of O’Neal and Gochman [C/in. Chem. 16, 985 (1970)] for determination of serum and urine amylase activityhas been modified to increase its accuracy, precision, and efficiency. Sampling rate is 50 specimens per hour. The analysis depends on ct-amylase hydrolyzing glycogen to maltose-like subunits, which are measured with the copper neocuproine reaction. Results obtained by ...
متن کاملAn automated, saccharogenic method for determining serum amylase activity.
An automated adaptation of the Somogyl saccharogenic determination of serum amylase is described in which conventional AutoAnalyzer modules are used. Adequate sensitivity with short incubation is achieved by incorporating glucose oxidase and catalase in the substrate to destroy serum glucose during incubation. Maltose and other dialyzable oligosaccharides are measured with the alkaline copper-n...
متن کاملFactitiously low amylase values.
When a commercial substrate was used in the determination of serum amylase activities of patients with acute pancreatitis, results much higher than those reported by the manufacturer with similar patients were found. A failure to quantitate all of the reducing groups liberated during incubation may account for the lower results. A modified saccharogenic method has been developed, which, by incr...
متن کاملA photometric method for the determination of alpha-amylase in blood and urine, with use of the starch-iodine color.
The use of the starch-iodine color for the estimation of ar-amylase appears to be on a sound theoretical basis. Swanson (1) has shown that in the degradation of amylose by a-amylase there is a random attack by the enzyme upon the polysaccharide chain yielding hexose units of varying lengths. Swanson (2) has further observed that chains 4 to 6 glucose units in length give no color with iodine, c...
متن کاملComparison of two methods for determining amylase activity in serum and urine.
E CLASSIC measurement of a-amylase (a1,4-glucan 4-glucanohydrolase, EC 3.2.1.1) activity in biological fluids is based on enzymatic hydrolysis of a starch substrate. This activity can be assessed either by a saccharogenic procedure (1, ), which measures the increase in reducing sugar (mainly maltose), or by an amyloclastic procedure (1, ), which depends on the decrease or disappearance of the b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 25 2 شماره
صفحات -
تاریخ انتشار 1979